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Related post: PROFESSIONAL: 3 OTHER: 1 CHECK APPROPRIATE BOX(ES) n (a) Human subjects H (b) Human tissues D (c) Neither D (a1) Minors D (a2) interviews SUMMARY OF WORK Buy Apcalis Sx (Use standard unreduced type. Do not exceed the space provided.) This project has been terminated. Pflii 6040 (Hev. b/92) 5-12-1 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER Z01A10057-19-LCI PERKDD COVERED October 1, 1991 to September 30, 1992 TITLE OF PROJECT (80 characlars or less. Tita must HI on ona Una between the borders.) Basic Studies on Pathogens Causing Cryptococcosis PRINCIPAL INVESTIGATOR (Ust other professional personnel below the Principal Invgstigalor.) (Name, title, latxyratory, and institute afliliation) K.J. Kwon-Chung, Research Microbiologist, Clinical Mycology Section, LCI, NIAID Others: Ashok Varma, Microbiologist, Clinical Mycology Section, LCI, NIAID COOPEFiATING UNITS (if any) LAB/BRANCH Laboratory of Clinical Investigation SECTION Clinical Mycology Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 20892 TOTAL STAFF YEARS: 2 PROFESSIONAL: 1 OTHER: 1 CHECK APPROPRIATE BOX(ES) D (a) Human subjects D (b) Human tissues Kl (c) Neither D (a1) Minors D (a2) Interviews SUMMARY OF WORK (Use standard unreduced type. Do not exceed Ihe space provided.) Genetic association of mating types and virulence for mice were found in Cryptococcus neoformans . A pair of congenic Cryptococcus neoformans var. neoformans strains, B-4476 (a^) and B-4500 (Ct) that presumably only differ in mating type, was constructed. This pair and their progeny, five OC type and five a_ type, were tested for virulence in mice. In the parent strains as well as the progeny, a type was clearly more virulent than a^ type. In addition, death tended to occur earlier among the mice infected with a strains than the mice that died by infection caused by a_ strains. All unstable URA 5 transf ormants (DNA mediated) of C. neoformans var. neoformans exhibited the presence of an autonomously replicating plasmid which had undergone significant sequence rearrangement. The autonomously replicating plasmids in the transf ormants were maintained in linear form and had acquired genomic sequences with homology to a sequence (s) on all the chromosomes. These results suggest mechanisms of plasmid maintenance in C. neoformans that Apcalis Sx 20mg are different from those reported for other yeasts. PHa 6040 (Hev. 5/52) 5-13-1 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl-Al 0058-18 LCI PERIOD COVERED October 1, 1991 to September 30, 1992 TITLE OF PROJECT (80 characlers or less. Title must Ht on ore line between the txrdars.) Pathogenesis and Chemotherapy of Herpesvirus Infection in Man PRINCIPAL INVESTIGATOR (List other prolassional personnel below the Principal Investigator) (Name, title, laboratory, and institute affiliation) PI: S.E. Straus - Senior Investigator - LCI, NIAID J. Cohen - Senior Staff Fellow - LCI, NIAID R. McKenzie - Medical Officer - LCI, NIAID R. Kost - Medical Staff Fellow - LCI, NIAID T. Heineman - Medical Staff Fellow - LCI, NIAID J. Meier - Medical Staff Fellow - LCI, NIAID P. Krause - Medical Staff Fellow - LCI, NIAID J. Dale - Researc h Nurse - LCI. NIAID COOPERATING UNITS (if any) J. Rooney, Senior Staff Fellow, LOM, NIDR; A. Notkins, Chief, LOM, NIDR; L. Stanberry, University of Cinncinnati LAB/BRANCH Laboratory of Clinical Investigation SECTION Medical Virology Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 20892 TOTAL STAFF YEARS: 4.6 PROFESSIONAL: 3.6 OTHER; 1.0 CHECK APPROPRIATE BOX(ES) Kl (a) Human subjects Kl (b) Human tissues D (c) Neither □ (a1) Minors D (a2) Interviews SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.)
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